click chemistry tools. CLICK AF-Dyes are routinely used for Click Chemistry – based labeling whereas NHS Ester or Maleimide-functionalized AF-Dyes react with primary amine or thiol modified biomolecules, respectively. Atto 488 NHS ester BioReagent, suitable for fluorescence, ≥80% (coupling to amines); find Sigma-Aldrich-41698 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Trafficking of internalized NGFR/p75 receptor with p75 antibody clone 8J2, conjugated with ATTO 488 (M-1821-50-AT, green). 1 mg. $105. DBCO-C6-NHS Ester. AxisPharm is a dedicated special chemical provider. Meineke et al. 1115-1. Cy7 Alkyne. DNA is an ultra-high-density storage medium that could meet exponentially growing worldwide demand for archival data storage if DNA synthesis costs declined sufficiently and random access of files within exabyte-to-yottabyte-scale DNA data pools were feasible. 1336-1. AFDye 350 NHS Ester. TAMRA Alkyne. MB 660R Alkyne. For each labeling reaction, use 100-500µL of purified protein sample at 1-2.5mg/mL. I need to buy an amine reactive dye and I was thinking to buy the alexa fluor 647 NHS ester. (C) Representative confocal microscopy images of epithelial cells expressing Muc1-42TR ΔCT showing actin staining with Alexa Fluor 568 phalloidin. 1 mg. $139. AFDye 647 Alkyne. Just wondering due to the stark price difference between AFDye 594 Cadaverine (Click Chemistry Tools) and Alexa Fluor 594 Cadaverine (TFS). 1 mg. $95. Recombinant His-tagged Podocalyxin was conjugated with Alexa Fluor 568 NHS Ester (Thermo Fisher Scientific), and the degree of labelling quantified according to the manufacturer’s protocol. click chemistry tools. engineer and combine mutually orthogonal Pyrrolysyl-tRNA-synthetase/tRNA pairs to expand the genetic code of mammalian cells with two noncanonical amino acids. Instagram, Twitter, Facebook, Images, Youtube and more on IDCrawl - the leading free people search engine. Shop a large selection of products and learn more about Click Chemistry Tools AFDye 647 NHS Ester | 25mg AFDYE 647 NHS ESTER 25MG . Carboxyrhodamine 110 Alkyne. We now offer both functionalized AF-Dyes and AF-Dye – nucleotide conjugates. I have reacted alexa fluor 647 NHS ester … 英文名称:DBCO-C6-NHS Ester. Cy5.5 Alkyne. At AxisPharm, we strive to provide our clients with special chemicals that are of the highest possible quality. 5-TAMRA Alkyne. We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. click chemistry tools. Buffers that contain primary amines (e.g., Tris or glycine) will interfere because they react with the NHS-ester moiety. Description: Carboxylic acid; also available as NHS-Ester, maleimide and conjugated to streptavidin. 全国客服热线(7*24小时) 全部产品分类. AFDye-647 NHS ester (cat#1121-100) and sulfo-cyanine3 NHS ester (cat#1301) were purchased from Fluoroprobes. CAS:1384870-47-6. 免疫学 疾病检测 科研检测 The bottom row shows the region of interest from the composite image (yellow box), n = 3. Cy7 NHS Ester. 1338-1. OG 488 Alkyne. click chemistry tools. Domains; ; has server used (United States) ping response time 31 ms Average ping Hosted in 1&1 IONOS Inc. Register Domain Names at 1&1 IONOS SE.This domain has been created 4 years, 276 days ago, remaining 89 days.You can check the number of websites and blacklist ip address on this server NHS-Ester, prepare the protein in phosphate-buffered saline to avoid precipitation. Cy5 NHS Ester. Expert Manufacturer Years of experience synthesizing fluorescent probes, from a few milligrams to hundreds of grams Quality & Reliability - Identical Compounds - Equal Performance - Consistent Results Selection Largest selection of functional groups and dyes available off-the-shelf. AF-Dyes (also known as Alexa Fluor® dyes) are hydrophilic fluorescent dyes with remarkable photostability. AFDye 568 NHS Ester. 由北京百奥创新科技有限公司提供,产品特点:北京百奥创新代理Click chemistry tools在中国的业务。Click chemistry tools授权代理,Click chemistry tools全国代理,Click chemistry tools北京代理,Click chemistry tools华东代理,Click chemistry tools华北代理。 Mucins are labeled with Alexa Fluor 647 PNA. 1 mg. $139. These data repeat and elaborate on (Figures 6A and 6B). 1342-1. Dissolve protein directly in the labeling buffer. click chemistry tools. Find Alexa Dye online. The reaction mixture was protected from light and was completed in 30 min. NHS ester functionalized dyes were used to conjugate with secondary antibodies. These two bioorthogonal chemical handles, encoded by distinct codons, allow selective, site-specific dual color fluorescent labeling and chemical-controlled crosslinking of surface receptors on living cells. 免疫学 疾病检测 科研检测 The absorbance range is 346-749 nm, and the emission range is 442-775 nm. Briefly, 40 μl of secondary antibody, 5 μl of 1 M NaHCO 3, and 1 to 2 μg of fluorophore were mixed. Afdye 647 maleimide fluoroprobes fluorescent labeling hilyteᵀᴹ fluor 647 c2 maleimide 247145 38 6 alexa fluor 568 nhs ester AFDye 594 Alkyne. AF Dyes (Alexa NHS Ester) Category Fluorescent Dyes Modification Code AFDye Reference Catalog Number 26-6XXX 5 Prime Y 3 Prime Y Internal Y Molecular Weight(mw) 695.6 This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is … 1076-1. Cells were again washed with PBS and labeled with 10 μM picolyl azide dye (AFDye 647 Picolyl Azide, Jena Bioscience), in 50 μM CuSO 4, ... (PEG 4-NHS ester)-Cy5 (N-Cy5-NHS, BroadPharm) was coupled with tetrazine amine (SiChem) at 2:1 molar ratio in PBS at 30°C for 42 h. A 200 μM stock solution was prepared in 100 mM Tris-HCl pH 8.0. Cy5 Alkyne. 全国客服热线(7*24小时) 全部产品分类. AF Dyes (similar to Alexa Fluor NHS Esters) are a set of fluorescent dyes that span the visible electromagnetic spectrum, as well as some of the near-IR. Cy3 Alkyne. AFDye 488 NHS Ester. 1 mg. $105. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. ... AFDye 568 Alkyne.


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